Read e-book online Advances in Enzymology and Related Areas of Molecular PDF

By Alton Meister

ISBN-10: 0470122641

ISBN-13: 9780470122648

ISBN-10: 0470648120

ISBN-13: 9780470648124

Advances in Enzymology and similar parts of Molecular Biology is a seminal sequence within the box of biochemistry, supplying researchers entry to authoritative reports of the most recent discoveries in all parts of enzymology and molecular biology. those landmark volumes date again to 1941, supplying an unequalled view of the historic improvement of enzymology. The sequence bargains researchers the newest realizing of enzymes, their mechanisms, reactions and evolution, roles in advanced organic procedure, and their software in either the laboratory and undefined. each one quantity within the sequence positive aspects contributions through major pioneers and investigators within the box from worldwide. All articles are conscientiously edited to make sure thoroughness, caliber, and readability.

With its wide selection of themes and lengthy old pedigree, Advances in Enzymology and comparable parts of Molecular Biology can be utilized not just by way of scholars and researchers in molecular biology, biochemistry, and enzymology, but additionally through any scientist attracted to the invention of an enzyme, its houses, and its purposes

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Extra info for Advances in Enzymology and Related Areas of Molecular Biology, Volume 19

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While malic acid is not a precursor of carbohydrates in plants, it neverthelew appears to he an important, photosynthetic product. (2) Isocitric Dehydrogmse (“lsocitric” Enzyme) A fixation of COz similar to the one which takes place in the “malic” enzyme reaction occurs in the synthesis of isocitric acid. The isocitric dehydrogenase system was shown to catalyze the reversible reaction (30) (250). Synthesis of d-isocitric acid, like thc COOH + Cot + TPNH + H + (Mn++)bHCOOH + TI”+ ;€I* Hz LO COOH LOOH a-Ketoglutaric acid AH* (30) bHoH LOOH Iaocitric acid synthesis of L-malic acid, can be brought about by coupling the isocitric dehydrogenase system (reaction 30) to the oxidation of G-6-P (reaction 25), as expressed in equation (31): G-6-P - + a-ketoglutarate- + COs + H20 TPN.

The behavior of “malic enzyme” is not duplicated by a mixture of malic dehydrogenase and oxalacetic carboxylase, at least not to the extent of catalyzing the reductive carboxylation of pyruvate (cf. especially Herbert (152)). TPN-specific “malic enzyme” is widely distributed in living beings. It has been found in animal tissues, including bird liver (267), mammalian liver (267), heart, brain, and kidney, as well as in Escherichiu coli and other microorganisms (261,262). Of special interest, because of its possible function in photosynthesis, is its widespread occurrence in the tissues of higher plants (368), particularly leaves.

Tetrachloro-o-quinone H1, tetrachlorocatechol. Ferricyanide H, ferrocyanide H f . . . . . Ferricytochromef H, ferrocytochromeI H+ p-Quinone Hz, hydroquinone.. . . . . . . . Ferricytochrome c H, ferrocytochromec H+ 2,6-Dichlorophenolindophenol Ha, leuco dye. . Methemoglobin H, hemoglobin H+. . . . 1,4-Naphthoquinone Hn,hydroquinone deriv.. Dehydroascorbate Hz, amorbate.. . . . . . Methylene blue Hz, leuco dye.. . . . . . . Ferric oxalate H, ferrous oxalate H+.

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Advances in Enzymology and Related Areas of Molecular Biology, Volume 19 by Alton Meister

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